The cell yield using Nycodenz concentrations at 8, 9.6, and 11% was evaluated by cell counting (A). Enrichment in HSCs cells was determined by cytofluorimetry (D) for desmin-positive cells as quantified in (B). The amount of contaminating cells (Kupffer and endothelial cells) was determined measuring the mRNA expression levels of clec4f and pecam1 genes, as markers of Kupffer and endothelial cells (C) after normalizing to the housekeeping gene Gapdh. Data are shown as means ± SEM, n=3, *P<0.05. Abbreviations: clec4f, C-type lectin domain family 4 member F; pecam1, platelet endothelial cell adhesion molecule-1.