HPLC analysis of ALA and LA in authentic standards (A) and PFO (B). The samples were derivatized with the mixture of Br-MMC and 18-crown-6 to produce a fluorogenic methyl-7-methoxycoumarin fatty acid derivative, fractionated on the C18 type column, eluted with a mobile solvent (acetonitrile:DI), and detected the fluorescence. ALA and LA in PFO concentrations were determined from their standard curves.