Y79 and WERI-Rb-1 cells were transfected with NC-siRNA or siRNA-KCNQ1OT1 for 48 h. (A) The proliferation of Y79 and WERI-Rb-1 cells was detected by CCK-8 assay. (B,E) The cell apoptosis and cell cycle of Y79 and WERI-Rb-1 cells were tested using Flow Cytometry. (C,D,F–M) The expression of CDK2, cyclinD1, pro-caspase3, cleaved-caspase3, MMP2, MMP9, N-Cadherin, vimentin, and cadherin expression in Y79 and WERI-Rb-1 cells were assayed using Western blot analysis. β-actin is a loading control. (N) The migration of Y79 and WERI-Rb-1 cells was tested using Transwell assay. *P<0.05 (vs. NC-siRNA), **P<0.01 (vs. NC-siRNA), ***P<0.001 (vs. NC-siRNA).