Figure 7
![HUVEC and HMEC-1 cells were treated with 800 μM CoNPs in combination with Fe(CH3CHOHCOO)2, Fe(CH2COO)2, or FeCl2. Fe2+ and total iron levels were measured following the CoNPs treatment. (A) Expression of HIF-1α, VEGF and GLUT1 were tested using Western blot (B) or PCR assay (C). Treatment with these three agents increased Fe2+ and total iron levels in HUVEC and HMEC-1 cells that were exposed to CoNPs. All three ferrous agents inhibited increase in HIF-1α, VEGF, and GLUT1 induced by CoNPs. **P<0.01 and ***P<0.001 vs. CoNPs group. Abbreviations: FC, ferrous chloride, (FeCl2); FL, ferrous lactate, [Fe(CH3CHOHCOO)2]; FS, ferrous succinate, [Fe(CH2COO)2].](https://port.silverchair-cdn.com/port/content_public/journal/bioscirep/41/1/10.1042_bsr20203456/1/bsr-2020-3456i007.png?Expires=1717071072&Signature=eokTrszcTpxr5aRo7XTBXtJ7Kp7cvUl~AND1uv0Pth3zhQuYg72zjWtLGwwWeAuEtmeYWNIKhjqOrQwJL6klnVDt7B18LTkulUe6tyJsw3RcAxcyQ0TMDP6VwVgQkdRbTVTZvpNBFmmD-vMf4YnhAV4P8MtzKnCMqwCU-23bUhOO0Kf9SrSxsbSDoJ~Qo38EbpRfrG8B7yeNhSM~IDVeGRufGeI6yqo5PEuB2U7U~Ev7kW62xO4lylE~xs6Xz5EbcjBnbgAQ7dtuKTQVrRfH6X0ciFx0P0key0EMfkhIMC1D8JFCEv37bWALftXdkIduHLay1RijOSMTwj2s-5UuBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The regulatory effects of ferrous agents on HIF-1α signal and Fe2+ levels in vascular endothelial cells treated with CoNPs
HUVEC and HMEC-1 cells were treated with 800 μM CoNPs in combination with Fe(CH3CHOHCOO)2, Fe(CH2COO)2, or FeCl2. Fe2+ and total iron levels were measured following the CoNPs treatment. (A) Expression of HIF-1α, VEGF and GLUT1 were tested using Western blot (B) or PCR assay (C). Treatment with these three agents increased Fe2+ and total iron levels in HUVEC and HMEC-1 cells that were exposed to CoNPs. All three ferrous agents inhibited increase in HIF-1α, VEGF, and GLUT1 induced by CoNPs. **P<0.01 and ***P<0.001 vs. CoNPs group. Abbreviations: FC, ferrous chloride, (FeCl2); FL, ferrous lactate, [Fe(CH3CHOHCOO)2]; FS, ferrous succinate, [Fe(CH2COO)2].