Figure 5
![HUVEC and HMEC-1 cells were treated with 800 μM CoNPs in combination with Fe(CH3CHOHCOO)2, Fe(CH2COO)2, or FeCl2. MTT (A), apoptosis (B) and LDH (C) assays were performed 24 h after the treatments. All three ferrous agents improved cell viability and decreased the apoptosis and the LDH concentrations in culture medium. **P<0.01 vs. control group; #P<0.05 vs. CoNPs group. Abbreviations: FC, ferrous chloride, (FeCl2); FL, ferrous lactate, [Fe(CH3CHOHCOO)2]; FS, ferrous succinate, [Fe(CH2COO)2].](https://port.silverchair-cdn.com/port/content_public/journal/bioscirep/41/1/10.1042_bsr20203456/1/bsr-2020-3456i005.png?Expires=1717043252&Signature=QT1Xi3~nPR96qEdzyOyGsLVIwI3AxhjUzN-etrTpKN2JJ9Vs38WXJ5SkLERSJYvwgvp0FJAn6Woq536fh-toQZfTZjydZwzaQCTeEWWvu6qoEhXFkLKuA~iz3su4nhInE9qiFnaEv~8WxLvYuVtUz7CXY7yzDTKBeGLU9hZqYSLncUXkMejeQIac~En3fkq8W7vqh2Rk96YMUABtDQ7TRjIzqk5dVPipdkOFIBY93SBjV4Ht3mYijJ3lSqXSotPmAeL-sZyv8kdcVuiPIfMZvOL7Gv8Cmr8V-RpRh4E4mmK~iMgR7pxb9mxNdNsEWG9MaQ9~tbidVrKQ0qXlQpDy5w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ferrous agents conferred protection against the toxicity of CoNPs in vascular endothelial cells
HUVEC and HMEC-1 cells were treated with 800 μM CoNPs in combination with Fe(CH3CHOHCOO)2, Fe(CH2COO)2, or FeCl2. MTT (A), apoptosis (B) and LDH (C) assays were performed 24 h after the treatments. All three ferrous agents improved cell viability and decreased the apoptosis and the LDH concentrations in culture medium. **P<0.01 vs. control group; #P<0.05 vs. CoNPs group. Abbreviations: FC, ferrous chloride, (FeCl2); FL, ferrous lactate, [Fe(CH3CHOHCOO)2]; FS, ferrous succinate, [Fe(CH2COO)2].