FtsE self-interaction was performed using SEC and glutaraldehyde cross-linking. Analytical size-exclusion chromatogram for FtsE in the absence (A) and the presence of ATP (B) are shown. (C) The SDS/PAGE image, is showing glutaraldehyde cross-linking of FtsE in the presence and absence of γ-ATP. (D) The SDS/PAGE showing glutaraldehyde cross-linking of FtsE in the presence of FtsZ and γ-ATP. (E) Y2H experiment showing the FtsE self-interaction.