The ATPase activity and ATP-binding studies were performed as described in the method section. (A) Shows the ATPase activity of wildtype FtsE and the two mutant versions of FtsE (EC49A and EK41A). (B) ATP-binding affinity of FtsE, was performed using fluorescence binding assay. (C) Graph showing the fluorescence intensity of MANT-ATP in the presence of wildtype FtsE and ATP binding mutant of FtsE (K41A). (D) Dendrogram is showing the relative fluorescence intensity of wildtype and mutant FtsE. (E) Job’s plot showing the binding ratio of FtsE and ATP (Mant-ATP used for fluroscence excitation).