miR-27b suppressed BC growth, while EN2 re-introduction reversed the inhibitory role of miR-27b in vivo
(A and E) Pictures of the typical mice and tumours after the mice received the inoculation for 40 days. BC cells with miR-27b-OE transfection reduced the tumour size, while EN2-OE transfection reversed the inhibitory role of miR-27b (*P<0.05 vs. the group miR-NC+EN2-OE; #P<0.05 vs. the group miR-NC+EN2-NC; &P<0.05 vs. the group miR-27b-OE+EN2-OE). (C) The typical pictures of xenograft tumour sections with haematoxylin stained by eosin. (D) The representation of protein of EN2 expressed in xenograft tumours by immunohistochemistry. The cytoplasm included most of the positive stains of EN2 and the number of cells with positive features was greater in the group miR-27b-OE+EN2-OE than in the group miR-27b-OE+EN2-NC. (F) The representation of miR-27b and EN2 genes expressed in transplanted tumours by qRT-PCR (*P<0.05 vs. other groups). (G and H) The representation of the protein of EN2 expressed in transplanted tumour using Western blot (*P<0.05 vs. other groups).