(A) The binding sites of miR-409-3p in SCAI 3′-UTR. (B) MiR-409-3p regulated the transcriptional activity of SCAI as detected by dual-luciferase reporter gene assay. (C) Relative expression of SCAI was detected by RT-qPCR in miR-409-3p analog and cs groups (vs. C analog/cs). (D) Relative expression of SCAI was detected by Western blotting in miR-409-3p analog and cs groups (vs. C analog/cs). *P<0.05, **P<0.01 (*, analog group; #, cs group). Abbreviations: C analog, control analog; C cs, control complementary sequence; miR-409-3p cs, miR-409-3p complementary sequence; NS, not significant.