(A) miR-409-3p expression was identified on 7th and 21st days of osteoblastic differentiation of MSCs. (B) ALP activity, (C) mineralization nodules in analog and cs groups on days 7 and 21. (D) Western blot shown the up/down-expression in above two groups of osteogenic marker proteins (ALP, OCN, OPN, RUNX2) (vs. C analog/cs). */^#P<0.05, **/^##P<0.01 (*, analog group; #, cs group). Abbreviations: C analog, control analog; C cs, control complementary sequence; miR-409-3p cs, miR-409-3p complementary sequence; NS, not significant.