Macrophage cells were seeded overnight at 500000 cells/well before subsequent treatment with 300 nM of toxin for 4 h. Samples were exposed for 40 ms at 358 nm for DAPI and 100 ms at 495 for FITC. Data were analyzed using ImageJ. (A) C3larvinA WT; (B) C3larvinA ΔY2-A31; (C) C3larvinA ΔY2-W34; (D) C3larvin_trunc; (E) C3larvinA ΔY2-W34 K36E. Co-localization of C3larvinA and C3larvin_trunc with cellular RhoA. Mouse macrophage J774A.1 cells were seeded overnight at 250000 cells/ml before treatment with 300 nM of toxin conjugated with Dylight 488 for 4 h. Samples were exposed for 40 ms at 358 nm for DAPI, 100 ms at 495 nm for FITC, and 100 ms at 532 nm for TRITC. Data were analyzed using ImageJ. (F) C3larvinA-treated cells and (G) C3larvin_trunc-treated cells.