Transfection efficiency expressed as percentage of GFP-positive cells by 15:1-CS3 (S) and (T) (graph A and dot plot A’) and 15:1-CS5 (S) and (T) (graph B and dot plot B’) co-complexation polyplexes with 100 µg of NLS peptides. Transfection was performed with 1 µg of DNA for all groups and analyzed 72 h after transfection. N=3, bars correspond to SD. Statistical differences compared with CS polyplexes (condition 0 in both graphs) were calculated using Dunnett's multiple comparisons test (****P<0.0001).