Size (graph A) and zeta Potential (graph B) of CS3 and CS5 polyplexes. The white bar represents the CS polyplexes without NLS. The graphs are representative for S and T formulations, as no differences were found between these formulations. Polydispersity was similar for all samples (below 0.4). Statistical differences, compared to polyplexes without NLS peptides (0) were calculated using Dunnett's multiple comparisons test (N=3, error bars = SD; **P<0.01; *P<0.05).