Effects of GDL treatment on the differentiation of NSCs in vitro
Immunostaining with Tuj1, MAP2, GFAP in NSCs treated with GDL, penicillamine, and GDL plus penicillamine (A–D). In addition, mRNA levels of Tuj1, MAP2, and GFAP in NSCs treated with GDL, penicillamine, and GDL plus penicillamine were determined using quantitative real-time polymerase chain reaction (E–G). *P<0.05, **P<0.01, compared with control.