Figure 3.
![(A) Coomassie Blue stained PAGE of fractions from a glycerol gradient that reveals formation of a Ctf4 dependent factory complex reconstituted from CMG, Pol ε, Ctf4, Pol α-primase. (B) Quantitation of the bands in lane 10 of Panel A. (C) Averaged EM images of mixtures of either, Top: CMG, Ctf4 and Pol α-primase. Bottom: CMG and Ctf4. (D) Cryo-EM model of 2CMG–Ctf4 (left, top) and cryo-EM density of CMG–Pol α-primase (left, bottom), and superimposition of the 2CMG–Ctf4 and Ctf4–Pol α-primase models. Panels are adapted from figures/movies in [30].](https://port.silverchair-cdn.com/port/content_public/journal/biochemsoctrans/48/6/10.1042_bst20200640/1/bst-2020-0640c.03.png?Expires=1716452504&Signature=RSSR1DNLgFEwTS9ed2Hi4R3qNmn3sPm6VkdgoPx4F3jq-~6bbyeiT8etl29z4lByPmdmAr-ExXR6EXgHOha7wuWREXEAOh2Wh6Vv0cmBvEUoRGo8RDIDKWoz5MAd5~7YGIZOEi31wvstEYO2ONkYufcaIBhVNKGJUzuMxieLfgOuZnUpFYi06ZV7NACys2MKPh9vNz8UjwKofEQGePLJKmNIv~c0vOvvr91U5Sm79OlmPgj8XTKX9~WtWp2mPCZfUefVA3YU~OY87khfqe7R9vmPscY1hNatPVyrYtq-1MkTOIrbDvOPJ37wG3OmDWZODKxASG-EWZ9Zvs9EVKliuw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reconstitution and visualization of a putative replication factory.
(A) Coomassie Blue stained PAGE of fractions from a glycerol gradient that reveals formation of a Ctf4 dependent factory complex reconstituted from CMG, Pol ε, Ctf4, Pol α-primase. (B) Quantitation of the bands in lane 10 of Panel A. (C) Averaged EM images of mixtures of either, Top: CMG, Ctf4 and Pol α-primase. Bottom: CMG and Ctf4. (D) Cryo-EM model of 2CMG–Ctf4 (left, top) and cryo-EM density of CMG–Pol α-primase (left, bottom), and superimposition of the 2CMG–Ctf4 and Ctf4–Pol α-primase models. Panels are adapted from figures/movies in [30].