![The exogenous κ-elastin enhances the mRNA expression of eNOS in the ECs [73], and tropoelastin improves the protein expression for NO production, which is inhibited by L-NAME [74]. The TSP-1-mediated inhibition of NO synthesis and downstream effects is through two main mechanisms: (1) The high concentrations of TSP-1 and its peptide mimetics, ABT-510, via interaction with CD-36, inhibit the translocation of myristate to the cytoplasm, thus inhibit the membrane translocation of the Src responsible the activation of eNOS and generation of NO [68,70]. (2) At low concentration, and via its binding to CD47, TSP-1 inhibits the NO-mediated activation of sGC, cGMP generation from Guanosine triphosphate (GTP), and activation of cGMP-dependent protein kinase (cGK-1) responsible for the enhanced cell proliferation, angiogenesis and metastasis [71,72]. The exogenous NO at a concentration of 1 µM only can decrease the protein expression of TSP-1 in the ECs, while activates sGC [67]. The scheme was created in Biorender.com.](https://port.silverchair-cdn.com/port/content_public/journal/biochemsoctrans/48/6/10.1042_bst20200208/1/bst-2020-0208c.02.png?Expires=1717006499&Signature=N5JjqaqcRUgAd5PDtZJCvUnW~S-GcyQm0uxJT4AQqfd~ppFiMs9RmFOmvhXJ-UyoNp2y2JiAb2a085fS8nVTPjRy97Zfpa4MyYsYqcmNagSO2h4vc5V-z0tgb7l9mW4exP~hAfmIPpgA-EzA3hUSXH2H7z4hYMwTdkyrfyVMn-tP95NtzjvcI0M~9spTndo5fQcZrl3RRHb2ZHE6gG2CgBLKR~gZsxVK1HURTDNY8K7yx-QOPvoKkSmXyLo5Fg2acDVLZBKIzcEA-zInexl78p2ZqQh935EST7~iU-W24NGXGHtzF58ODkz9hCsSxGoQK9Uo60SMvITN1EyibG5Fnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The exogenous κ-elastin enhances the mRNA expression of eNOS in the ECs [73], and tropoelastin improves the protein expression for NO production, which is inhibited by L-NAME [74]. The TSP-1-mediated inhibition of NO synthesis and downstream effects is through two main mechanisms: (1) The high concentrations of TSP-1 and its peptide mimetics, ABT-510, via interaction with CD-36, inhibit the translocation of myristate to the cytoplasm, thus inhibit the membrane translocation of the Src responsible the activation of eNOS and generation of NO [68,70]. (2) At low concentration, and via its binding to CD47, TSP-1 inhibits the NO-mediated activation of sGC, cGMP generation from Guanosine triphosphate (GTP), and activation of cGMP-dependent protein kinase (cGK-1) responsible for the enhanced cell proliferation, angiogenesis and metastasis [71,72]. The exogenous NO at a concentration of 1 µM only can decrease the protein expression of TSP-1 in the ECs, while activates sGC [67]. The scheme was created in Biorender.com.