Figure 3
Three groups were divided here, namely, TNBC cell line, respective GRC line and GRC treated with NLRP3 inhibitor CY-09. (A,C) RT-PCR evaluated the relative mRNA expression of E-cadherin and Vimentin in all the groups. (B,D) ELISA methods measured the IL-1β secretion, p-GSK3β-Ser9 and β-catenin protein expression so as to monitor the activity of Wnt/β-catenin signaling pathway. All the assays were performed thrice. **, P<0.05.
NLRP3 antagonist inhibited EMT, IL-1β and Wnt/β-catenin signaling pathway in gemcitabine-resistant TNBC in vitro

Three groups were divided here, namely, TNBC cell line, respective GRC line and GRC treated with NLRP3 inhibitor CY-09. (A,C) RT-PCR evaluated the relative mRNA expression of E-cadherin and Vimentin in all the groups. (B,D) ELISA methods measured the IL-1β secretion, p-GSK3β-Ser9 and β-catenin protein expression so as to monitor the activity of Wnt/β-catenin signaling pathway. All the assays were performed thrice. **, P<0.05.

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