(A) Gross hearts from the indicated groups at 5 weeks after sham or TAC surgery, n=5-6 mice per group, scale bar, 2 mm. (B) Representative image of H&E staining from the indicated groups, n=6 mice per group, (scale bar, 25 µm). (C) Comparison of the CSA of cardiomyocytes from the indicated groups. (D–F) Real-time PCR analysis of mRNA expression levels of hypertrophic marker genes (Anp, Bnp, Myh7), n=4 mice per group. (G,H) Protein expression levels (G) and statistical quantification (H) of the hypertrophy marker proteins (ANP, BNP, MYH7), n=4 mice per group. The GAPDH level was used as the internal control in (D–H). *, P<0.05; **, P<0.01; n.s., no significance, P>0.05.