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Figure 6

(A) Dose–response curve for HeLa WT and HeLa HAX1 KO cells treated with increasing H2O2 concentrations. Dots on the graph represent normalized results obtained from four independent MTT assays. Gray ribbons represent 95% confidence intervals. Significance assessed with Welch’s two sample t-test for each H2O2 concentration (350, 450, 550, 650, 750, respective P-values: 0.5021, 4.75E-09, 1.33E-12, 9.20E-13, 1.43E-08). (B) In situ protein aggregates detection. Aggregates stained with Proteostat, nuclei stained with Hoechst 33342. Scale bar: 10 μm. (C) Violin and box plots representing quantification of intracellular protein aggregates detected in HeLa WT and HeLa HAX1 KO cells. For each cell line protein aggregates from ≈200 cells from three independent experiments were quantified. Difference between cell lines was assessed by Mann–Whitney U test (P-value <2.2e-16).

HAX1 knockout affects a response to oxidative stress and protein aggregation

(A) Dose–response curve for HeLa WT and HeLa HAX1 KO cells treated with increasing H₂O₂ concentrations. Dots on the graph represent normalized results obtained from four independent MTT assays. Gray ribbons represent 95% confidence intervals. Significance assessed with Welch’s two sample t-test for each H₂O₂ concentration (350, 450, 550, 650, 750, respective P-values: 0.5021, 4.75E-09, 1.33E-12, 9.20E-13, 1.43E-08). (B) In situ protein aggregates detection. Aggregates stained with Proteostat, nuclei stained with Hoechst 33342. Scale bar: 10 μm. (C) Violin and box plots representing quantification of intracellular protein aggregates detected in HeLa WT and HeLa HAX1 KO cells. For each cell line protein aggregates from ≈200 cells from three independent experiments were quantified. Difference between cell lines was assessed by Mann–Whitney U test (P-value <2.2e-16).

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