Western blotting of protein expression in tumor tissue
(A–G) Refers to the expression bands and respective histograms of rhythm- and drug-resistance related proteins in tumor tissue. (A) Shows the protein-expression bands in the four groups. Histogram of (B) PER2, (C) Akt, (D) PI3K, (E) MDR1, (F) DYRK2 and (G) MPR1. Western blotting showed that expression of Akt, PI3K, MDR1 and MRP1 in tumor tissue injected with SKOV3/DDP cells was higher than that in tumor tissue injected with SKOV3 cells (DYRK2 expression was lower). Expression of Akt, PI3K and MDR1 in tumor tissue exposed to a circadian-rhythm disorder was higher than that in tumor tissue exposed to a normal circadian rhythm (DYRK2 expression was lower) and the difference was statistically significant (P<0.01). Expression of Per2 in SKOV3/DDP cells was lower than that in SKOV3 cells, whereas expression of Per2 in dysrhythmic tumors was lower than that in normal tumors, and all differences were statistically significant (P<0.01).