Activation of TRPV2 induces rapid translocation in leading edges but not on the filopodial tips
(A) Live cell imaging of F11 cell co-transfected with TRPV2-GFP and actin-RFP shows that activation of TRPV2 by Probenecid alters actin cytoskeleton dynamics leading to changes in cell morphology, such as extension of cell membrane (upper panel), formation of massive lamellipodia (middle panel) and merging of lamellipodia (lower panel). (B) Activation of TRPV2-GFP by 2APB (indicated by green arrow at 886th frame) also causes changes in submembranous actin cytoskeleton and results in rapid translocation of TRPV2-GFP to the leading edges, merging of actin-ribs in the lamellipodium boundary. Intensity profile of the TRPV2-GFP and actin-RFP are provided below. (C) Enlarged section of the leading edge of a F11 cell expressing TRPV2-GFP and actin-RFP before and after activation with 2APB. The filopodial tips are marked with white arrows. TRPV2-GFP is mainly present in the filopodial base but not in the filopodial tips.