(A) TRPV2 co-localizes with Phalloidin in filopodia and specific cellular areas. (B) Live cell image of F11 cell co-expressing TRPV2-GFP (green) and actin-RFP (red) demonstrating co-localization of TRPV2-GFP and actin-RFP in specific cellular regions such as in neurites and in filopodia. (C) The C-terminus of TRPV2 interacts with actin. Soluble brain extract supernatant (lane 1) was pulled down with purified MBP-LacZ (lanes 4–5), MBP-TRPV2-Ct (lanes 6–7) and was probed for bound actin by Western blot analysis.