(A) Immunofluorescence images of F11 cells stained with anti-TRPV2 antibody in the absence (lower panel) or presence (upper panel) of specific blocking peptides are shown. (B) Western blot analysis of F11 cell extract probed with anti-TRPV2 antibody are shown. The presence of specific blocking peptide diminished the TRPV2-specific immunoreactivity completely. (C) Live cell imaging of F11 cells incubated with Fluo-4 demonstrating the transient and sharp increase in the intracellular Ca2+-level immediately after treating the cells with a specific activator (Probenecid, 250 µM). The interval between each time frame is 0.5 s. (D) Similar Ca2+-imaging of F11 cells shows an immediate drop in intracellular Ca2+- levels followed by application of specific inhibitor (Tranilast, 75 µM). Further application of specific activator (Probenecid, 250 µM) causes a sudden increase in Ca2+-level. (E,F) Quantification of intracellular Ca2+-levels as shown above (C,D) are represented. In each case, fluorescence intensity (in arbitrary units) from multiple cells (n=10) are shown. The average value is shown as a thick black line. The interval between each time frame is 0.5 s.