Fecal DNA was extracted from wild-type mice and Hfe^−/− mice and 16S metagenomic sequencing was performed to determine the bacterial composition in two animal groups. (A) The distribution of five Proteobacteria classes in the wild type and Hfe^−/− mouse fecal material. (B) Species enrichment analysis in the wild type and Hfe^−/− mouse fecal material. Data show mean values for six mice per group of the gut microflora components whose changes were statistically significant.