Expression of HIF-2α and DMT1 in wild type and Hfe−/− mouse colon.
(A) HIF-2α expression was monitored in colon at the protein level by Western blot with β-actin as an internal control. (B) DMT1 (divalent metal ion transporter 1) expression was monitored in colon as well as in the duodenum at the mRNA level by qPCR with GAPDH mRNA as an internal control. ns, not significant; *P < 0.05.