Iron overload in Hfe−/− mouse colon, serum, and liver.
(A) Total iron (Fe+2 and Fe+3) concentration in wild-type mouse colon and Hfe−/− mouse colon. (B) Heme concentration in wild-type mouse colon and Hfe−/− mouse colon. (C) Total iron (Fe+2 and Fe+3) concentration in serum of wild-type mice and Hfe−/− mice. (D) Total iron (Fe+2 and Fe+3) concentration in liver of wild-type mice and Hfe−/− mice. Data show mean values (±SEM) for three mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.