Portland Press

Figure 2.

Mitochondrial proteins were incubated at either 15°C or 30°C in a buffer containing 200 mM Tris–HCl, pH 7.5, 10 mM MgCl2, 10 mM CaCl2, 50 mM ATP and 0.1% Triton-X100 for the indicated time periods (0, 4, 8, 12 and 24 h). Samples were separated by SDS–PAGE, transferred to a polyvinylidene difluoride membrane and incubated with antibodies to AOX. AmAOX positions are indicated by arrows. Molecular mass standards are also indicated.

Degradation of AmAOX proteins in mitochondria purified from appendices at various developmental stages in A. maculatum.

Mitochondrial proteins were incubated at either 15°C or 30°C in a buffer containing 200 mM Tris–HCl, pH 7.5, 10 mM MgCl₂, 10 mM CaCl₂, 50 mM ATP and 0.1% Triton-X100 for the indicated time periods (0, 4, 8, 12 and 24 h). Samples were separated by SDS–PAGE, transferred to a polyvinylidene difluoride membrane and incubated with antibodies to AOX. AmAOX positions are indicated by arrows. Molecular mass standards are also indicated.

This Feature Is Available To Subscribers Only