(A,B) Level of MTDH in NSCLC tissues and cell lines was detected by qRT-PCR. SPC-A1 and A549 cells were transfected with NC, miR-126-5p, miR-126-5p+vector, or miR-126-5p+MTDH, (C) and MTDH expression level was examined by Western blot. (D) The proliferation abilities of SPC-A1 and A549 cells were analyzed by CCK-8 assay. (E) Apoptotic cells were analyzed using flow cytometry. (F,G) Transwell assay was performed to assess cell migration and invasion in NSCLC cells. (H,I) The expression levels of EMT-relative proteins, including E-cadherin, N-cadherin, and Vimentin were identified via Western blot assay. *P<0.05.