miR-219a-5p regulates radiosensitivity in NSCLC cells
(A,B) Radioresistant NSCLC cell lines were generated using gradually increasing doses of irradiation in A549 and H358 cells. The sensitivity of radiosensitive and radioresistant A549 and H358 cells was examined using irradiation at 0, 2, 4, 6, 8 and 10 Gy, at a dose rate of 2 Gy/min. (C) Relative level of miR-219a-5p in A549 and H358 parental and radioresistant cells. (D,E) Radioresistant A549-RR and H358-RR cells were transfected with miR-219a-5p and then irradiation-induced cytotoxicity was determined using CCK8 assay. (F,G) Parental radiosensitive A549-RS and H358-RS cells were transfected with anti-miR-219a-5p and then irradiation-induced cytotoxicity was determined using CCK8 assay, *P<0.05.