C57BL/6 mice were anesthetized and hair was removed from the dorsal surface area (A). A 4-mm full thickness excisional skin wound was created on the dorsal area (B). A sterile band-aid was placed so that the wound was centered within it, and a transparent film dressing (Tegaderm 3M) was placed over the wound using an immediate-bonding adhesive to better fix the dressing to the skin (C).