Artesunate inhibited osteoclastogenesis through the miR-503/RANK axis
RAW264.7 cells were treated with RANKL (100 ng/ml) in the presence of 8 μM artesunate with or without miR-503 inhibitor. (A) The osteoclast differentiation assay was performed. The number of TRACP-positive multinucleated cells was counted; Scale bars: 500 µm. The arrows in the diagram represent the formation of TRACP-positive osteoclasts. (B) The mRNA expression levels of NFATc1, TRAP, and cathepsin k were measured by qRT-PCR. Data are presented as the mean ± SD from three independent experiments; *P<0.05, **P<0.01, ***P<0.001.