Effect of MCM3 knockdown on CRC cell cycle distribution and apoptosis
(A) The cell cycle distribution of COLO205 cell was detected by FCM. (B) The results of FCM measurement showed that the cell cycle was arrested in G1 phase after the MCM3 knockdown. (C) Apoptosis ratios of MCM3 in stably transduced sh-Ctrl and sh-MCM3 COLO205 cells were detected by FCM analysis. (D) The protein expression of CDK2, CDK4, CyclinD1 and CyclinE1 was significantly inhibited after MCM3 knockdown; CRC, colorectal cancer; FCM, flow cytometry. All representative data are from three independent experiments; *P<0.05, **P<0.01.