(A) The mRNA level of MCM3 in different CRC cell lines from CCLE analysis. (B) The mRNA expression of MCM3 in COLO205 and normal colon mucosal epithelial cell was detected by qRT-PCR (n=3 independent experiments **P<0.01). (C) The protein levels of MCM3 in stably-transduced sh-Ctrl and sh-MCM3 COLO205 cell line were analyzed by Western blot (n=3 independent experiments **P<0.01); CCLE, cancer cell line encyclopedia; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction.