Successfully constructed glioma stable cell line that knocks down hnRNPA2/B1
(A) hnRNPA2/B1 after 24 h of transfection. (B) hnRNPA2/B1 transfected for 2 weeks and screened by G418 (×200). (C) RT-qPCR analysis of hnRNPA2/B1 mRNA expression levels in the transfected group (*P=0.0142, <0.05 vs control, analyzed by t test). (D,E) Western blot analysis of hnRNPA2/B1 protein expression levels in the transfected group, blank group (cells with only transfection reagent), sh-control group (transfected with control shRNA); and sh-hnRNPA2/B1 group (transfected with sh-hnRNPA2/B1 vectors). Data were based on at least three independent experiments and are shown as the mean ± SD (*P=0.0188, <0.05 vs control, analyzed by t test).