(A) FBXW7 has a potential binding site with miR-223-3p. (B and C) Dual luciferase reporter gene results in two RCC cell lines. (D and E) FBXW7 was down-regulated in RCC tissues at protein level. (F) The transfection efficiency of miR-223-3p mimic or inhibitor in 786-0 cells. (G) FBXW7 protein expression is down-regulated after miR-223-3p increment in 786-0 cells, whereas it is elevated after down-regulation of miR-223-3p. (H) The transfection efficiency of miR-223-3p mimic or inhibitor in ACHN cells. (I) FBXW7 protein is decreased after up-regulation of miR-223-3p in ACHN cells, while it is increased after down-regulation of miR-223-3p. (J) The expression of FBXW7 is pronouncedly decreased in renal cancer cell lines. (K) The expression of FBXW7 and miR-223-3p are notably negatively correlated in renal cancer tissues. (L) FBXW7 is prominently positively correlated with RASA1 expression in renal cancer tissues; *P<0.05, **P<0.01.