(A) In 786-0 cells, the expression of RASA1 is tested by qRT-PCR. (B) The expressions of RASA1 and apoptosis index Caspase-3 are detected by Western blot. (C) Flow cytometry is employed to examine the apoptosis rate after overexpression of RASA1. (D) In ACHN cells, qRT-PCR assay verifies the overexpression efficiency of RASA1. (E) Western blot is applied to examine the expressions of RASA1 and Caspase-3. (F) Flow cytometry is adopted for examining the apoptosis rate after overexpression of RASA1; *P<0.05.