(A) qRT-PCR manifests that RASA1 is expressed at lower levels in RCC tissues than in paracancerous tissues. (B) Through examination, the expression levels of RASA1 in six RCC cell lines are found to be reduced relative to those in HK-2 cell line. (C) RASA1 was down-regulated in RCC tissues at protein level. Data are presented as mean ± SD in three independent assays; *P<0.05, ***P<0.001.