(A) The binding sites of miR-96-5p on NAMPT 3′-UTR were predicted by Targetscan software. (B and C) The luciferase activity was detected in HEK293T and RAW264.7 cells co-transfected with miR-96-5p and NAMPT-WT or NAMPT-MUT. (D and E) The mRNA levels of NAMPT in RAW264.7 cells transfected with miR-96-5p mimic or anti-miR-96-5p were detected by qRT-PCR. (F) The protein levels of NAMPT in RAW264.7 cells transfected with miR-96-5p mimic or anti-miR-96-5p were detected by Western blot analysis; *P<0.05.