LC-MS analysis of PydAc-catalyzed uracil reduction with ferredoxin as the reductant
EIC (m/z 115) chromatograms are given to detect the presence of product dihydrouracil (theoretical m/z = 115). (A) LC-MS elution profiles of authentic standards of dihydrouracil, negative controls omitting ferredoxin and uracil and a complete assay are displayed as labeled. (B) ESI (+) m/z spectra of the dihydrouracil peak in complete assay and coincidence with theoretical m/z of dihydrouracil.