In IL-4 treated THP-1-differentiated macrophages, pSin-GNAS-AS1 plasmids or negative plasmids were transfected into cells, then next experiments were conducted. (A) The level of GNAS-AS1 was evaluated using qRT-PCR. (B) Flow cytometry was used to quantify the proportion of M1 or M2 macrophages. (C) qRT-PCR was performed to determine the expression levels of M2 macrophage markers (IL-10, Arginase-1). (D) CCK-8 assay was conducted to assess the cell viability of T47D and MCF-7 cells co-cultured with above treated THP-1-differentiated macrophages. (E) Wound healing assay was used to examine the migration of T47D and MCF-7 cells co-cultured with above treated THP-1-differentiated macrophages. (F) Transwell was applied to detect the invasion of T47D and MCF-7 cells co-cultured with above treated THP-1-differentiated macrophages. Data with error bars are presented as the mean ± SD. The Student’s t test and one-way ANOVA test were used to determine significance; *P<0.05, **P<0.01, ***P<0.001.