(A) The relative quantity of GNAS-AS1 in ER⁺ breast cancer tissues and adjacent normal tissues was examined by qRT-PCR. (B) Human monocytes were isolated from PBMCs with antibody against CD14 and CD11b and analyzed using flow cytometry. (C) Flow cytometry was used to quantify the proportion of M1 or M2 macrophages. (D) M1 macrophage markers (TNF-α, IL-6) were measured by qRT-PCR. (E) M2 macrophage markers (IL-10, Arginase-1) were detected by qRT-PCR. (F) qRT-PCR was performed to determine GNAS-AS1 expression in TAMs (M0, M1, M2). (G) The expression level of GNAS-AS1 in breast cancer cells (T47D and MCF-7) and normal mammary epithelial cells (MCF10A) were determined by qRT-PCR. Data with error bars are presented as the mean ± SD; *P<0.05, **P<0.01, ***P<0.001 as determined by the Student’s t test or one-way ANOVA test.