Nascent CL is deacylated by Cld1 in yeast; phospholipase A₂ (PLA₂) in mammals. Tafazzin transfers an acyl chain from another phospholipid (PL) to MLCL, forming CL and a lysophospholipid (LPL). After one or more cycles, this will result in the mature form of CL. CL acyltransferase enzymes, which take MLCL and acyl-CoA as substrate, are also shown: acyl-CoA:lysocardolipin actyltransferase 1 (ALCAT), monolysocardiolipin acyltransferase 1 (MLCLAT), and trifunctional protein alpha subunit (TFPα). Enzyme names are coloured green and lipid names are in black.