Figure 1.
The OnGuard2 Arabidopsis model was used, as described by Wang et al. [8], without alteration (WT) and after eliminating the H+-ATPase dependencies on light (-L), on [Ca2+]i (-C), and on both (-LC). (A–C) shows the steady-state current–voltage curves for the two K+ channel currents sampled at midday. The inward-rectifying K+ channel current is evident at voltages negative of −120 mV while the outward-rectifying current is visible at voltages positive of −60 mV. The WT curve (black lines) is reproduced in each frame for comparison with the ost2 component deconstruction (grey lines). (D) Maximum conductance (gmax) for the outward-rectifying K+ channel in (A–C) for each of the four-parameter combinations as determined by fitting to a Boltzmann function (see [72]). (E) Maximum conductance (gmax) for the inward-rectifying K+ channel in (A–C) for each of the four-parameter combinations as determined by fitting to a Boltzmann function (see [72]). (F) Range of stomatal conductance (gs) calculated from OnGuard2 as described by Wang et al. [8] for each of the four-parameter combinations in (A–C). Dashed lines are included for reference to the WT model.
Deconstruction of the Arabidopsis ost2 mutant in constitutive activation of AHA1, the dominant H+-ATPase of the guard cell plasma membrane.

The OnGuard2 Arabidopsis model was used, as described by Wang et al. [8], without alteration (WT) and after eliminating the H+-ATPase dependencies on light (-L), on [Ca2+]i (-C), and on both (-LC). (AC) shows the steady-state current–voltage curves for the two K+ channel currents sampled at midday. The inward-rectifying K+ channel current is evident at voltages negative of −120 mV while the outward-rectifying current is visible at voltages positive of −60 mV. The WT curve (black lines) is reproduced in each frame for comparison with the ost2 component deconstruction (grey lines). (D) Maximum conductance (gmax) for the outward-rectifying K+ channel in (AC) for each of the four-parameter combinations as determined by fitting to a Boltzmann function (see [72]). (E) Maximum conductance (gmax) for the inward-rectifying K+ channel in (AC) for each of the four-parameter combinations as determined by fitting to a Boltzmann function (see [72]). (F) Range of stomatal conductance (gs) calculated from OnGuard2 as described by Wang et al. [8] for each of the four-parameter combinations in (AC). Dashed lines are included for reference to the WT model.

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