(A) A cell model of KLF15-overexpressed podocyte was established. After treatment of adenovirus packing ORF–KLF15 clone, KLF15 mRNA expression was obviously increased in podocyte. (B) Consistent with mRNA expression, the protein expression of KLF15 was obviously increased in adenovirus packing ORF–KLF15 clone treated podocytes. (C) In KLF15 overexpressed podocytes, the mRNA expression of Bax, a well-recognized indicator of apoptosis, was significantly decreased as the anti-apoptotic Bcl-2 mRNA was increased. (D) Consistently, Bax protein expression was down-regulated while Bcl-2 protein expression was up-regulated in KLF15 overexpressed podocytes. (E and F) Podocytes were stained with APC/PI for flow cytometry analysis. Compared with Ad-GEP podocytes, the cell apoptosis rate was significantly reduced in Ad-KLF15 podocytes. (G–L) The flow cytometry analysis showed apoptosis induced by ADR, LPS or HG was decreased in Ad-KLF15 podocytes. (M) Quantitative results of apoptosis rate. Data were from at least three independent experiments; *P<0.05 and **P<0.01 vs. controls.