Nar enhanced the cytotoxicity of MTX against HepG2 cells
Cells were treated with increasing concentrations of Nar and/or MTX (12.5 μM–3.2 mM) for 48 h. Controls were treated with DMSO only. (A) The effects of MTX and/or Nar on the proliferation of HepG2 cells are expressed as a percentage of cell viability. (B) A semilogarithmic plot of the cell viability percentage of HepG2 cells over the 48 h exposure to increasing concentrations of MTX and/or Nar. IC50, absolute IC50 is the concentration that corresponds to 50% inhibition of the viability of HepG2 cells. Data from at least three independent experiments performed in at least triplicate are presented as the means ± SEM, *P<0.01 vs. control group.