(A) Representative results of SIRT1 immunohistochemistry in myocardial tissue. (B) SIRT1 expression in nuclei in myocardial tissue detected using Western blot analysis. (C) mTOR expression in myocardial tissue measured by Western blot analysis. The data are expressed as the mean ± standard deviation, n=6. One-way ANOVA and Tukey’s multiple comparisons test were applied to determine statistical significance. *P<0.05, compared with the sham group, #P<0.05, compared with the MI/R group.