Targeting relationship of miR-101-3p and PTGS2 determined based on bioinformatics prediction and luciferase activity assay
(A) qRT-PCR was used to detect the expression of miR-101-3p and PTGS2 in each group. (B) Western blot assay was used to detect the protein expression of PTGS2 in each group. (C) Binding sites between miR-101-3p and PTGS2 were predicted by bioinformatics analysis. (D) Dual luciferase reporter assay analysis. The data analysis was performed using one-way ANOVA, followed by pairwise comparison using LSD-t. The experiment was repeated three times. aP<0.05 vs. the mimic-NC group; bP<0.05 vs. the sh-NC group; cP<0.05 vs. the miR-101-3p inhibitors + sh-NC group.