(A) Quantitative PCR analysis of Abcg2 mRNA in colon epithelium of Hfe^−/− mice and wild-type mice. Data show mean values of three mice per group ± SEM relative to control mice. **P < 0.01. (B) Western blot for Abcg2 protein levels in ileal and colonic epithelium from wild type and Hfe^−/− mice. Please see Supplementary Figures S13 and S14 for full blot images. Western blot band intensities were estimated using ImageJ software and the band intensities that correspond to the Abcg2 protein were normalized to the respective β-actin levels in (C) ileal epithelium and (D) colonic epithelium. Data represent mean values of three mice per group ± SEM. **P < 0.01.