(A) Quantitative PCR analysis of Glut9 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values ± SEM relative to liver, which is taken as 1. *P < 0.05; ***P < 0.001. (B) Western blot for Glut9 protein levels in liver and intestinal segments of the wild-type mouse. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective β-tubulin band intensities. Please see Supplementary Figures S5 and S10 for full blot images. (C) Quantitative PCR of Glut9 mRNA in liver, jejunum, ileum, and colon of Hfe^−/− mice and wild-type mice. Data show mean values of three mice per group ± SEM relative to control mice. (D) Western blot for Glut9 protein levels in Hfe^−/− mouse and wild-type mouse liver, jejunum, ileum, and colon. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective β-actin band intensities. Please see Supplementary Figures S8, S9, and S11 for full blot images. (E) Quantitative PCR analysis of Mrp4 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values ± SEM relative to liver, which is taken as 1. *P < 0.05; ***P < 0.001. (F) Quantitative PCR analysis of Mrp4 mRNA in liver, jejunum, ileum, and colon of Hfe^−/− mice and wild-type mice. Data show mean values of three mice per group ± SEM relative to control mice. **P < 0.01.