TRIB2 was negatively correlated with miR-125b-5p in LSCC cells
Notes: MiR-NC, miR-125b-5p, miR-125b-5p+vector, or miR-125b-5p+TRIB2 vector was separately transfected into AMC-HN-8 or M4E cells as four groups. (A and B) Western blot assay and qRT-PCR was used to detect TRIB2 expression in transfected cells. (C and D) CCK-8 assay was conducted to assess cell proliferation. (E and F) The flow cytometry assay was performed to detect cell apoptosis rate in transfected cells. (G and H) The migration and invasion were assessed by Transwell assay in AMC-HN-8 and M4E cells in four groups; *P <0.05.